| ハイライト: | Optimized Nitrogen Substrate Peptone,Advanced Fermentation Processes Chicken Bone Peptone,Balanced Peptide Profile Nitrogen Substrate |
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Specialty Chicken Bone Peptone is a scientifically formulated nitrogen source produced from fresh poultry bone stock through a multi-stage enzymatic hydrolysis process. This product delivers a balanced peptide profile specifically optimized for demanding microbial fermentation systems, vaccine production platforms, and specialized culture media formulations. Its consistent nutrient composition supports reproducible biomass accumulation and metabolite production across various bacterial and fungal strains.
| Attribute | Specification |
|---|---|
| Base Material | Fresh chicken bone from USDA-inspected facilities |
| Collection Protocol | Temperature-controlled processing within 4 hours of slaughter |
| Quality Criteria | Free from pathological tissue, chemical residues, and environmental contaminants |
| Supply Chain | Documented traceability from source to finished product |
| Processing Module | Technical Parameters |
|---|---|
| Initial Processing | Cryogenic grinding, thermal sanitation, fat separation |
| Hydrolysis System | Sequential enzyme treatment with endo- and exopeptidases |
| Clarification Train | Depth filtration → activated carbon treatment → membrane filtration (0.45μm) |
| Evaporation Stage | Falling film evaporation at ≤60°C |
| Drying Technology | Multi-stage spray drying with particle size classification |
| Quality Verification | Real-time NIR monitoring + comprehensive batch testing |
Peptide Distribution – Optimized molecular weight profile (500-3000 Da) enhances microbial uptake efficiency
Mineral Balance – Controlled cation/anion ratio minimizes precipitation in media formulations
Thermal Stability – Maintains solubility and nutrient availability through standard sterilization cycles
Lot Uniformity – Statistical process control ensures CV <5% for key parameters across production batches
Endotoxin Control – Low endotoxin levels suitable for sensitive biological applications
| Parameter | Test Method | Unit | Specification Range | Lot Analysis |
|---|---|---|---|---|
| Total Organic Nitrogen | Kjeldahl (GB 5009.5) | % | 14.0 – 15.5 | 14.8 |
| Primary Amino Groups | Formol titration | % | ≥ 2.5 | 2.9 |
| Chloride (as NaCl) | Argentometric (GB/T 12457) | % | ≤ 5.0 | 2.3 |
| Residual Moisture | Gravimetric (GB 5009.3) | % | ≤ 6.0 | 4.2 |
| Incineration Residue | 550°C (GB 5009.4) | % | ≤ 6.0 | 4.5 |
| Aqueous pH | Potentiometric (2% solution) | pH units | 5.2 – 6.8 | 5.6 |
| Optical Density (620nm, 2% soln) | Spectrophotometric | Absorbance | ≤ 0.10 | 0.06 |
| Nitrite Content | Diazotization (GB 5009.33) | mg/kg | ≤ 2.0 | < 1.0 |
| Total Heavy Metals | ICP-MS | mg/kg | ≤ 10 | < 10 |
| Bulk Density | Tapped density | g/mL | 0.45 – 0.65 | 0.52 |
| Microorganism | Test Protocol | Performance Indicator | Result |
|---|---|---|---|
| Salmonella enterica serovar Typhi | H₂S production (lead acetate method) | Positive control response | + (48h) |
| Shigella dysenteriae | H₂S production (lead acetate method) | Negative control response | - (48h) |
| Escherichia coli (ATCC 25922) | Indole production (Kovac's reagent) | Positive response | + (24h) |
| Salmonella enterica serovar Typhi | Indole production (Kovac's reagent) | Negative response | - (24h) |
| Enterobacter cloacae | Voges-Proskauer reaction | Acetoin production | + (24h) |
| Escherichia coli (ATCC 25922) | Voges-Proskauer reaction | Acetoin production | - (24h) |
| Streptococcus pneumoniae | Blood agar hemolysis | Alpha pattern (green) | Stable through 3 passages |
| Streptococcus pyogenes | Blood agar hemolysis | Beta pattern (clear) | Stable through 3 passages |
| Pseudomonas aeruginosa | Pigment expression (King A medium) | Pyocyanin production | Green pigment stable |
| Staphylococcus aureus | Pigment expression (nutrient agar) | Staphyloxanthin production | Golden pigment stable |
| Escherichia coli | Colony morphology (2% peptone agar) | Diameter, colony type | ≥1.2mm, 98% smooth |
| Staphylococcus aureus | Colony morphology (2% peptone agar) | Diameter, colony type | ≥1.0mm, 97% smooth |
| Streptococcus pyogenes | Growth sensitivity (dilution series) | Maximum supporting dilution | 10⁻⁴ |
| Bacillus subtilis | Growth sensitivity (dilution series) | Maximum supporting dilution | 10⁻⁵ |
| Application Field | Recommended Concentration | Performance Benefit |
|---|---|---|
| Antibiotic Fermentation | 1.5 – 2.5% (w/v) | Enhanced secondary metabolite production |
| Enzyme Production | 2.0 – 3.0% (w/v) | Balanced C:N ratio supports protease/amylase yield |
| Vaccine Manufacturing | 1.0 – 2.0% (w/v) | Consistent antigen expression profiles |
| Diagnostic Media | 1.0 – 1.5% (w/v) | Clear background, no interfering reactions |
| Probiotic Cultivation | 1.5 – 2.0% (w/v) | High viable cell recovery |
| Research Media | Variable | Reproducible growth curves across experiments |
| Component | Specification |
|---|---|
| Primary Container | Food-grade polyethylene liner, heat-sealed |
| Secondary Packaging | Multilayer kraft paper bag with moisture barrier |
| Unit Size | 20 kg net weight (±0.1 kg) |
| Pallet Configuration | 40 bags per pallet (800 kg), stretch-wrapped |
| Labeling | Product name, lot number, net weight, manufacture date, storage conditions |
Optimal Storage: 15 – 25°C, ≤50% relative humidity
Shelf Life: 24 months from manufacture date
Stability Indicators: Retain sealed until use; protect from moisture, heat, and direct light
Post-Opening Handling: Use within 30 days after opening; reseal tightly after each use
| Aspect | Details |
|---|---|
| Manufacturing Standard | ISO 9001:2015 certified facility |
| Product Specification | Q/SCSH 003-2025 (Chicken Peptone, Biological Grade) |
| Reference Documents | Chinese Biological Products Raw Material Standards (2000) |
| Analytical Methods | GB, ChP, and EP referenced procedures |
| Documentation | Full batch traceability, COA available for each lot |
For technical consultation, sample requests, or customized formulations, please contact our biotechnology support team.